Detection of 2-Photon Oxidation from a NIR Laser Using Confocal Microscopy

摘要

Recent studies have determined that photochemical oxidation in cultured cells can be detected at peak irradiances as low as 8.5x10(8) W cm(-2) (87-fs pulse). Fluorescent dyes, such as CM-H(2)DCFDA, enable us to quantify the oxidation response of cells to mode-locked near-infrared (NIR) laser exposure. Using a modified confocal microscope, we characterize the time- dependent 2-photon induced fluorescence generated from a given NIR laser exposure. When cultured cells were then pre-loaded with antioxidants, ascorbic acid or N-acetyl-L-cysteine (NAC), they inhibit nonlinear oxidation with different efficiencies, providing insight regarding mechanisms of damage.