Integrated Development of Serum Molecular Markers for Early Diagnosis of Breast Cancer

摘要

We have identified two panels of SEREX antigens associated with breast cancer in pre- and post-menopausal women. Suspended bead arrays were generated using Luminex microspheres. Serum samples from patients with breast cancer, patients with benign breast lesions and healthy volunteers were collected and concentrations of serum breast cancer biomarkers samples were determined. Novel ADEPT bioinformatics algorithm was utilized to analyze the results. In the postmenopausal group, a 23-biomarker panel was selected using the projection pursuit technique correctly classifying 84% of the test set observations, with a sensitivity of 85% at a specificity of 83%. In the premenopausal group, a 20-biomarker panel was identified providing sensitivity of 88% at a specificity of 89% increasing that of mammography for this population. Parallel analysis of these samples using SELDI-TOF MS technology in combinations with BI-RADS scores allowed for correct classification of 80% of patients. The presented data demonstrate that simultaneous testing of a panel of breast cancer-associated serum antigens identified by SEREX using suspended bead array technology allows establishing comprehensive antigenic profiles with high predictive power for breast cancer.