Cell Motility and Invasiveness of Neurofibromin-Deficient Neural Crest Cells and Malignant Triton Tumor Lines. Addendum

摘要

Our purpose is to examine the role of the NF1 gene product neurofibromin in modulating the migratory and invasive properties of neural crest cells (NCC) and neural crest-derived sarcoma cells. As a negative regulator of Ras signaling neurofibromin may influence the responses of NC- derived cells to growth factors and extracellular matrix (ECM) molecules that affect motility. We have completed our analyses of Nf1-/- embryonic NCC invasiveness in vitro compared effects of neurofibromin deficiency in different embryonic mesenchymal cell populations derived from cranial and trunk regions and characterized expression of neural stem cell markers in the cell populations used. We have completed immunoblot analyses of POOF signaling in sarcoma lines derived from cisNf1 +1/-;p53+/- mice. Finally we have expanded our analyses of the cisNf1 +/-;p53+/- mouse model to characterize mutant frequency in normal and tumor tissues and to compare DNA repair capacities in neurofibromin-deficient Schwann cells and Schwann cell-derived tumor cell lines.