Rapid Detection & Identification of Bacillus Species using MALDI- TOF/TOF and Biomarker Database

摘要

A strategic plan to develop a matrix-assisted laser desorption/ionization with time-of-flight (MALDI-TOF/TOF) tandem mass spectrometry (MS) method in conjunction with a biomarker database for rapid microbial identification is described in this technical memorandum. The objective is to develop rapid, highly sensitive and definitive protocols for detection and identification of BW agents such as Bacillus species and demonstrate their - effectiveness. The protocols will be based on MALDI-TOF MS technology applicable to intact microorganisms. MALDI can directly analyze biological samples for biomarkers (proteins, lipids, etc.). Forensic biomarkers can be used to identify microorganisms at species and strain levels. In addition to method development, a new mass spectral biomarker database could be created and populated for selected species and strains, starting with selected Bacillus species. This technology would create a new area for comprehensive forensic analysis of biological materials that are not addressed by current methods (PCR and DNA sequencing,- and immunoassays). This memorandum contains a review of Bacillus species and current detection and identification methods, and a review of MALDI-TOF/TOF MS methods and biomarker databases.