Rapid Molecular Fingerprinting of Pathogens

摘要

The first aim of this project was to identify a set of ligands that bind specifically to each member of a panel of related alpha viruses, namely, equine encephalitis viruses (EEV). The second aim was to develop a bacterial cell-based array platform that can be used to detect viruses present in serum or other complex mixtures. During the course of this project we generated a set of peptides that bind to Venezuelan or Western EEV (VEEV, WEEV), and investigated a microfluidic array method to pattern these affinity reagent displaying cells into arrays, allowing their potential application for detection assays.