S-Layer Based Bio-Imprinting - Synthetic S-Layer Polymers.

摘要

The main objective of the proposed work is the development of a key enabling technology for the fabrication of nano patterned thin film imprints by using functional S-layer protein arrays as templates. The unique feature of these imprints is the precisely controlled repetition of surface functional groups and topographical features induced by the crystalline character of the reassembled S-layer protein lattice. The start of the project focused on the selection, composition and chemical modification of suitable polymers (Methacrylic acid (MAA), Vinylpyrrolidone (VP)) as well as on the preparation and reassembly of the S-layer protein SbpA from Lysinibacillus sphaericus CCM2177 on silicon supports that were used as stamps. First of all, the rigidity (expressed by the Young (elastic) modulus) of the S-layer with respect to that of the polymer was investigated in order to make sure that the Slayer is mechanically robust enough to leave an imprint behind. It was found that the Young modulus of the S-layer is ca. 7 times higher compared to that of the polymer.