Water Sparing in Chronic Ethanol Exposure is Associated With Elevated Renal Estrogen Receptor Beta and Vasopressin V2 Receptor mRNA in the Female Rate

摘要

Fluid handling is known to differ between males and females. Interactions between sex steroids such as estrogen with fluid regulating hormones like vasopressin (VP) are likely key to establishing these differences. Research has identified estrogen receptor (ER) alpha and Beta in renal tissue which may affect renal fluid handling. Thus, this study hypothesized that chronic ethanol exposure would elicit different alterations to water load excretion between male and female Sprague Dawley (SD) rats due to changes in renal VP V2 receptor (V2R) or ER mRNA expression. Therefore, in this study we compared 120 minute excretion of a 2% Body Weight (BW) water load between male control (n=6) and ethanol-fed (n=14) rats, and female control (n=26) and ethanol-fed (n=26) rats. Additionally renal papilla mRNA expression of V2R, ERalpha, and ERbeta was compared between male control (n=S) and ethanol- fed (n=I 5) rats, and female control (n=12) and ethanol-fed (n=17) rats. Female ethanol fed rats showed a 16% reduction in water load excretion.