Tissue Microarray Assessment of Novel Prostate Cancer Biomarkers AMACR and EZH2 and Immunologic Response to Them in African-American and Caucasian Men

摘要

Prostate cancer is characterized by complex molecular events influenced by diverse genetic and environmental factors. The objective of the present study was to compare the expression of AMACR and EZH2 in African- American patients and Caucasian patients with prostate cancer. We constructed 5 tissue microarrays representing 40 African-American and 159 Caucasian prostate cancer patients and performed immunohistochemistry on these arrays using antibody to AMACR and EZH2. Protein expression was scored on these arrays for both AMACR and EZH2 analyzed the data generated from these experiments to investigate the relative levels of two markers in prostate cancer patients from the two racial subgroups and also for any associations with survival patterns and clinic-pathologic parameters. The mean of AMACR expression percentage of PCA patients is significantly higher in Caucasian patients than African American patients with prostate cancer. The mean of EZH2 intensity score in CaucaPCA patients is not significantly different from the score in AA PCA patients.