Role of Stat3 Activation During Prostate Cancer Progression

摘要

The purpose of this proposal is to detect the role of Stat3 activation during prostate cancer progression. Amultifaceted approach is being used to accomplish the proposed research goals. Significant progress was made insupport of Task I in that we were able to overexpress activated Stat3 (Stat3C) in tow human prostate cell lines. Both lines were characterized and at least on line (MDAPLa 2b) exhibits molecular alterations consistent with Stat3 activation. In support of Task 2 we have begun characterization of the Pb.Stat3C transgenic line and the preliminary histopathological findings indicate that expression of activated Stat 3 may confer a neoplastic phenotype. The proposed bigenic cross (Pb.Stat3C x BK5.IGF-I) has also been initiated as well as an alternate strategy due to the limited fertility of the BK5.IGF-1 transgenic line and the apparent compromise in viability of the bigenic offspring. Our results to date have been promising and should further our understanding of the role of Stat3activation in prostate cancer progression.