CTL-Tumor Cell Interaction: The Generation of Molecular Probes Capable of Monitoring the HLA-A*0201-HER-2/neu Peptide Complex

摘要

The overall goal of this project is to probe the CTL - tumor cell interaction by generating scFv probes that are able to recognize the HLA-A*0201- HER-2/neu369-377 peptide complex. In the second 12-month funding period (Feb 2005 - Feb 2006) scFv 2.3.5-58-53 was isolated following site-directed mutagenesis in the VL CDR3 and VL CDR1 of HLA-A*0201 HER 2/neu369-377 complex- specific scFv 2.3.5. In the 12-month period covered by this report (Feb 2006 - Feb 2007), I aimed to further improve the recognition of HLA-A*0201-HER- 2/neu369-377 complexes by scFv 2.3.5-58-53. To this end an scFv-Fc construct was utilized in an attempt to improve the avidity of the scFv for HLA-A*0201 HER-2/neu369-377 complexes expressed on tumor cells. Although scFv 2.3.5-58-53-.