Prostate Expression Databases: Gene Expression Resources for Comparative Studies of Prostate Carcinogenesis

摘要

This proposal aims to test the hypothesis that integrating observations derived from mouse model systems with observations from human prostate cancers will define relevant and consistent molecular alterations critical to the development and progression of prostate carcinoma. The research accomplished to date has: (1) assembled the requisite mouse models to enable the generation of tumor gene expression data; (2) produced a second-generation mouse prostate microarray that will allow for deeper profiling of mouse prostate gene expression; (3) identified a specific gene (osteopontin) commonly associated with multiple mouse prostate cancer models; (4) developed the methods/techniques that will enable precise dissection of mouse prostate epithelium; (5) expanded the Prostate Expression Database to archive microarray data; (6) determined strain-specific gene expression differences in the mouse prostate that could contribute to phenotypic differences on prostate cancer development and progression; and (7) identified developmental pathways altered in the Pten-/- prostate cancer model that could contribute to the process of carcinogenesis.