Adoptive Immunotherapy Combined with Hematopoietic Cell Transplantation as a Therapeutic Approach to Treatment of Prostate Cancer

摘要

We determined that cell lysates prepared from canine prostate tissue was immunogenic when injected in female dogs. In addition to the known prostate antigen, canine prostate specific esterase (CPSE), we identified by molecular weight several other proteins against which the dog made IgG antibodies. Using a mixed lymphocyte reaction, we found that a cellular response was generated against the prostate cell lysate after the female dog was injected 3 times with antigen. Sensitization of female dogs with prostate antigen suspended in incomplete Freund's adjuvant appeared to be superior to prostate lysate antigen- loaded canine autologous dendritic cells when both were injected subcutaneously near the popliteal lymph node. Peripheral blood lymphocytes and lymph node- derived lymphocytes reacted to prostate lysate in the mixed lymphocyte reaction. An immune response to prostate lysate or CPSE was not adequately detected by a delayed-type hypersensitivity reaction 10 days after the last of three subcutaneous injections of antigen. Two of three male dogs transplanted with dog leukocyte identical female bone marrow engrafted, but additional time is need to ensure engraftment is stable before prostate antigen sensitized donor lymphocytes can be infused into the recipient for an anti-prostate immune response to be evaluated.