Proteomic Study of Human Malaria Parasite Plasmodium Vivax Liver Stages for Development of Vaccines and Drugs

摘要

GFP plasmids were constructed and used for transfection of blood and sporozoite stages of P. vivax. GFP transformed parasites were observed when transfection was performed using blood stage parasites. Transfection of GFP plasmids into liver cells could enhance plasmid uptake of the sporozoites after invasion of the liver cells. Optimization of transfection for the GFP plasmids to blood and sporozoite stages need to be further optimized. Partial proteomic of sporozoite were studied and some parasite proteins were found to be up- regulated at this stage. These proteins could be used as markers to follow the parasite development in the liver cells in vitro.