Targeted Therapies for Myeloma and Metastatic Bone Cancers; Annual rept. 18 Jan 2007-17 Jan 2008

摘要

Developed methods to radiolabel polymer nanoparticles, the first time to our knowledge this has been done. Quantified functional groups available for ligand conjugation using S35-labeled ligands. Developed alternative assay to confirm affinity of bone-targeting nanoparticles to hydroxyapatite substrates. Demonstrated in vitro stability of radiolabeled nanoparticles. Successfully transferred our nanoparticle preparation protocols to another facility to support in vivo biodistribution studies. Started in vivo biodistributions validating ability of our radiolabeled nanoparticles to be imaged for up to 48 hours and further confirming our protocol methods to study bone-targeting nanoparticle biodistribution via radio-imaging.