Anti-Inflammatory Cytokine II-10 and Mammary Gland Development

摘要

The purpose of the award is to compare mammary glands from wildtype and IL-10 knockout mice at critical stages of development. IL10-knockout mice were imported and maintained in University at Buffalo lab animal specific pathogen-free facility. During the first year of the funding (July 06-July 07), we established protocols in our lab for genotyping, mammary gland whole mount preparation and the preparation of paraffin sections of mammary glands. We then mated IL10+/- male with IL10+/- females to generate 144 experimental female mice with +/+ or -/- genotype (72 each) from 80 litters. After excising and processing the glands at 5 critical stages of mammary gland development as described in the grant, we developed data analysis methods to perform the planned comparison. There were no differences between the wildtype (IL10+/+) and IL10-/- mice at up to age day 55. At age day 80 and 150, IL10-/- mice had less terminal end buds suggesting an alteration in mammary gland development. Part of the results are presented in the 2008 Era of Hope meeting (meeting abstract attached).