Topical Application of Liposomal Antioxidants for Protection Against CEES Induced Skin Damage; Final rept. 1 Jul 2005-30 May 2008

摘要

This study was successful in developing an effective prophylactic therapy against skin damage caused by an analog of mustard gas, 2- chloroethylethyl sulfide (CEES) using in vitro model systems. The therapy is based on the topical application of antioxidant liposomes. Both EpiDerm cultured human skin tissues as well as cultured keratinocytes were used as the in vitro model systems. Our results show that CEES pathophysiology involves oxidative stress and liposomes containing both water- and lipid-soluble antioxidants are, therefore, likely to be effective therapeutic agents for protecting US military and civilians from a chemical warfare agent such as mustard gas (HD). In summary, we have determined the therapeutic efficacy as well as the chemical and physical stability of various antioxidant liposome formulations for future testing in animal models.