Keratinocyte Spray Technology for the Improved Healing of Cutaneous Sulfur Mustard Injuries

摘要

The purpose of the current research is to determine whether the spray-on application of allogeneic keratinocytes in suspension will improve epidermal wound healing of vesicating burns induced by the chemical warfare agent sulfur mustard (HD). A beige SCID mouse model is used for these experiments which are being carried out in two phases. The first phase is dose ranging. The second phase tests the efficacy of spray keratinocytes (Universal Donor) at healing HD injuries. To limit combined injuries previously observed with HD in methylene chloride, dose ranging was carried out using ethanol as diluent and HD delivered to the dorsum of depilated mice within an 8 mm diameter cloning ring. The minimal HD exposure required to generate confluent epidermal and follicular necrosis, thrombi, and inflammatory infiltrate was identified (80 micrometers HD in 25 micrometers ethanol). Both Universal Donor cells and the SF parent cells promoted healing of debrided HD wounds; engraftment was variable.