Proteomic Analyses of Cellular Events Mediating/Inhibiting Chemical- Induced Injury

摘要

This project used a proteomic approach consisting of two-dimensional electrophoresis and mass spectrometry to analyze differential protein expression in response to formalin, JP-8 jet fuel, and carbon nanoparticle exposure. The aims were to assess the effect of various chemical exposures both in vivo and in vitro as a means to determine the utility of proteomic approaches in chemical toxicologic investigation. The results suggest that a comprehensive proteomic approach that includes both electrophoretic and mass spectrometric analyses can provide mechanistic information regarding chemical effects, and that the approach can generate additional hypotheses regarding individual chemical effects for further investigation. This is particularly true when proteomic data is combined with toxicological endpoint data and functional analyses.