CHD8, A Novel Beta-Catenin Associated Chromatin Remodeling Enzyme, Regulates Androgen Receptor Mediated Gene Transcription

摘要

The activity of the androgen receptor (AR) is critical for normal prostate development and function, but AR activity also plays a major role in the development and progression of prostate cancer. To better understand the function of beta-catenin in AR mediated transcription, we have identified a novel chromatin remodeling enzyme, CHD8, that can associate with beta-catenin and functions in AR mediated gene transcription. Year 1 was focused on the first specific aim of this proposal; the interaction of CHD8 with beta-catenin and the androgen receptor. Experiments were performed to characterize this interaction in vitro using recombinant proteins, and reagents were made to characterize this interaction in vivo using chromatin immunoprecipitation techniques. This work is required for the study of the function of CHD8 and &(number sign)946;-catenin in AR mediated transcription and the ultimate understanding of the role played by CHD8 in prostate cancer progression.