Microbiology and Biodegradation: Deep Ultraviolet Microscopy for the Detection, Quantification, and Characterization of Microbes.

摘要

This proposal was funded for the improvement and use of a deep ultraviolet (DUV) fluorescence imaging (microscopic) system, and a coupled DUV Raman Spectrometer. This was to be used for the study of microbial cultures with several goals in mind: 1) to rapidly quantify microbes on surfaces of filters and minerals; 2) to distinguish different microbial species from one another; 3) to look for subtle changes in microbes as a function of growth conditions, and 4) to improve the fluorescence and Raman systems for higher resolution measurements. Goal 1 was accomplished with the DUV fluorescence system, and studies, and goal 2 was in the midst of its work when funding was discontinued. At this point, the instrument development (improvement of resolution) was stopped at the advice of the Program Manager (Dr. Kizer), and measurements of samples with regard to goals 1 and 2 were continued until the end of the funding period.