Detection of Prostate Cancer Progression by Serum DNA Integrity

摘要

Our objectives/hypotheses are obtaining additional diagnostic and prognostic insight through the analysis of tumor-related DNA integrity and methylation in prostate cancer patient sera, we are developing optimized, robust, and reproducible blood assays. In the past grant period we have been accruing patients, blood specimens. Currently we are optimizing assays and final validation. have moved forward to validate our initial findings on cell lines and tumor specimens in serum and plasma. The Alu qRT assay is being investigated using plasma. In a study of 57 prostate patients, we found significant differences of unmethylated LINE1 (uLINE1) status in patients compared to normal healthy male. The data is in final stage of manuscript preparation for manuscript submission. Assays for the biomarkers will be further carried out for the direct quantitative RealTime PCR (qRT) Alu and direct qRT LINE1 is being optimized. We will also continue to develop circulating DNA methylated GSTP1 assay to complement the DNA integrity biomarkers (Alu and LINE1). The final validation of the assay specimens will be completed and manuscript(s) will be submitted.