In Vivo Test for Chemical Induction of Micronucleated Polychromatic Erythrocytes for Mouse bone Marrow Cells, Test Article, Diethylene Triamine Trinitrate (DETN)

摘要

Diethylene triamine trinitrate (DETN) (100%) pure was tested for its potentials to induce micronucleated polychromatic erythrocytes (MPCE) in the bone marrow cells of male and female CD-1 mice according to OECD TG 474 in compliance with Good Laboratory Practice. Male and female mice were dosed orally at 500, 1000, and 2000 mg;/kg. Animals were euthanized approximately 24 and 48 hr after dosing. The percent of polychromatic erythrocytes (PCE) and frequency of micronucleated polychromatic erythrocyte (MPCE) were determined at 24 and 48 hrs. Cytotoxicity was assessed by scoring the number of PCE and norchromatic erythrocyte (NCE) in first 200 erythrocytes for each animal. There were no statistically significant increases in the number of MPCE in the treated groups when compared to the concurrent vehicle control group. These results indicated that DETN was negative in vivo mouse micronucleus assay.