Role of BRCA2 in the Expressions of IRF9-regulated Genes in Human Breast Cells.

摘要

We hypothesize that BRCA2 facilitates the formation and/or the function of the ternary ISGF3 complex and thus, functional BRCA2 protein is essential for the antiproliferative effects of type I interferons against human breast tumor cells. Specific aims: (A) To evaluate further the structural and functional interactions of BRCA2 with the members of the ISGF3 complex (STAT1, STAT2 and IRF9) in the human breast cells. (B) To evaluate the antiproliferative effects of BRCA2 over expression in the human breast cells with or without knock down of the IRF9 protein by RNA interference. (C) To evaluate the antiproliferative effects of type I interferons against tumors developed by BRCA2 positive and BRCA2 negative human breast tumor cells in the nude mice xenograft model.