Coordination of BRCA1/BARD1- and MRE11/RAD50/NBS1-Dependent DNA Transactions in Breast Tumor Suppression

摘要

The main objective of the proposal is to understand how the Mre11- Rad50-Nbs1 complex and the BRCA1-BARD1 complex interact on DNA and coordinate DNA transactions that are critical for the maintenance of genomic stability and to prevent breast tumor development. We have characterized the behavior of BRCA1/BARD1 on DNA, using a single molecule approach. We have developed new single molecule technologies to study the behavior of BRCA1/BARD1 and MRN complexes. Finally, we have established that MRN-BRAC1/BARD1 interactions are dispensable for MRN/CtIP dependent DNA end resection, the first step of homology-dependent repair of DNA double-strand breaks.