Participation of Bone Marrow-Derived Cells in the Formation of Tumor- Associated Stroma During Lung Cancer

摘要

Tumor-associated stroma is known to play a critical role in the formation and progression of lung tumors. This study was performed to evaluate the fate of bone marrow derived cells (BMDCs) in the normal and neoplastic lung tissues. We have successfully established several experimental protocols which are critical to pursue the main objective. These include protocols for lung cancer development using adenovirus and conditional knockout mice. BMDCs isolation and culture from mice transplantation of the BMDCs by irradiating mice. We confirmed the migration and distribution of BMDCs in the stroma of normal lung tissues. The fate of BMDCs in the lung tumor tissues are under investigation. Successful completion of the proposed study will provide a novel experimental system which can be used to study mechanisms that control the formation of the tumor-associated stroma, critical interactions between bone marrow-derived cells and tumor cells and/or resident stromal cells, and treatments that control stromal cells and, thus, tumor growth.