RESEARCH ON THE PRODUCTION AND IMMUNOLOGICAL EXAMINATION OF ARTIFICIAL ANTIGENS CONTAINING KNOWN SUGARS (OR OLIGO-SACCARIDES) AS THE DETERMINANT GROUPS, IN RELATION TO THE IMMUNOCHEMICAL ANALYSIS OF ENTEROBACTERIAL O-ANTI-GENS (ENDOTOXINS)

摘要

Main efforts were concentrated on: (1) Methods for the transformation of bacterial O-specific polysaccharide haptens into full antigens. Two methods were worked out, namely (a) Preparation of p-aminobenzyl ethers of the polysaccharides which were then diazotized and coupled to proteins, giving polysaccharide benzylazo proteins. (b) The polysaccharides were allowed to react with epichlorohydrine in slightly alkaline solution resulting in cross-linking of polysaccharide units to larger (soluble or insoluble) particles. (2) Studies on the influence of certain substituents after their introduction into the polysaccharide(s) with respect to the antigenicity of the artificial polysaccharide complexes. Special emphasis was put on the esterification of the polysaccharide component with long-chain fatty acids 'Lipoidisation'. Also the introduction of charged groups was included (phosphorylation). (Author)