Metabolites of Pinacolyl Methylphosphonofluoridate (Gd) Following Enzymatic Hydrolysis in Vitro

摘要

Analytical procedures for the characterization and quantitative estimation of phosphonic acids derived from GD, based upon paper chromatography and partition between isobutanol-benzene and an aqueous phase, were developed. By using P32-labelled phosphonic acids, as little as .01 micromole acid could be determined. Application of these methods to the enzymatic hydrolysis products of GD suggests that the principal, if not the only, metabolite of GD incubated with rat plasma or liver tissue in vitro is pinacolyl methylphosphonic acid. (Author)