Rapid Calibration of Microtitration Diluting Loops by Refractive Index Methods

摘要

A rapid and simple method for calibrating the wire diluting loop used in the microtitration method is described. Favorable comparisons of the wire loop, with accepted pipetting procedures, were obtained in making serum dilutions utilizing a refractive index technic. The diluting loop has to its advantage the rapidity of making from 3-6 simultaneous dilutions using only 0.025 ml volume increments. With an easy method of calibrating the loops, any laboratory should be able to use the micro method with the confidence that is now given the conventional glass pippeting method.