Sustained Release Oral Nanoformulated Green Tea for Prostate Cancer

摘要

We developed an HPLC method to determine the amount of EGCG encapsulated in the nanoparticles. HPLC analysis showed that chitosan nanoparticles can efficiently entrap EGCG. Also, we developed a method to extract EGCG from the plasma with a recovery value of > 95%. We determined the effect of oral nanoEGCG on the growth of tumors in athymic nude mice implanted with 22RV1 cells. Treatment with EGCG (40 mg/kg body wt.) and nanoEGCG (3 and 6 mg/kg body wt.) resulted in significant inhibition of tumor growth. In control group, the average tumor volume of 1,200 mm3 was reached in 32 days after tumor cell inoculation. At this time point, the average tumor volume was 514, 310 and 216 in EGCG, nanoEGCG (3mg/kg body wt.) and nanoEGCG (6mg/kg body wt.) treated groups, respectively. The average tumor volume of 1,200 mm3 was achieved 46, 53 and 60 days after tumor cell inoculation in EGCG, nanoEGCG (3mg/kg body wt.) and nanoEGCG (6mg/kg body wt.) treated groups, respectively. At 28 days post-inoculation, there was 36, 54 and 72% decrease in the secreted PSA levels in EGCG, nanoEGCG (3mg/kg body wt.) and nanoEGCG (6mg/kg body wt.) treated groups, respectively as compared to the control group.