Electron Microscopy Methods of Investigation of Biological Objects

摘要

Basic methods of investigating biological objects are outlined. Special features of biological preparations which impart characteristic features to the process of preparing specimens are examined. Methods of contrasting with the help of shadowing, dyeing with salts of heavy metals, and also the method of 'negative' contrasting are examined in detail. Various means of preparing replicas are described. The questions of fixation and dehydration of objects and the methodology of preparing ultrathin sections are examined in detail. Some practical problems of working with the electron microscope and questions of photographic technique are outlined briefly. (Author)