to Study Therapeutic Properties of Blood Plasma in the Therapy of Tourniquet Shock

摘要

Tests of therapeutic effectiveness of infusions of different human plasma fractions in the treatment of rat tourniquet trauma revealed a striking superiority of Cohn Fraction II in protecting against mortality of the trauma. The substitution of this human plasma fraction for a portion of the rat's own plasma protein in rat plasma markedly improved the therapeutic effectiveness of the rat plasma. Studies of hematocritis and fluid loss into the traumatized hind limbs revealed that animals treated with Fraction II infusions maintained a larger circulating plasma volume (lower hematocrit) than animals infused with albumin or saline. This was thought to be due to recruitment of fluid into the circulating volume combined with a smaller loss into the hind limbs. Chloramphenicol pretreatment of saline-treated traumatized animals did not produce any improvement in survival over the appropriate control group. Although infusion of recently collected human plasma did not allow any survival when used to treat the standard trauma, gamma globulin from 'fresh' plasma protected as well as did gamma globulin from aged plasma. This finding suggests that the improved portection found with aged plasma was related to the disappearance of deleterious properties from fresh plasma not associated with the gamma globulin. (Author)