Approximate Enzymic Method of Microanalysis for Identifying Residual Amounts of Organophosphorus Insecticides

摘要

A simple, highly sensitive group method of detecting phosphorus-containing insecticides in various objects of the surroundings, based on their inherent anticholinesterase action, was developed. In order to realize this method a portable kit was constructed which contains a rack for the storage of ampouled reagents. The kit contains enough reagents for 100 analyses. The detection of insecticides may be performed under field conditions. A preliminary determination of insecticide contamination of products may be obtained in 10 - 20 min. The anticholinesterase effect of 6 of the most important insecticides was studied and calibration curves were constructed for their determination. The method (without preliminary activation) makes it possible to determine from 1 to 100 micrograms of insecticide per 1 ml. It was established that the anticholinesterase activity of chlorphos when dissolved in buffer solutions of pH 7.4 and under the action of plant extracts gradually increases. This is evidently explained by a conversion of the insecticide into a compound possessing high anticholinesterase activity. (Author)