NITROGENOUS SUBSTANCE CHANGES IN THE NUTRITIVE BROTHS PREPARED FROM THE DRY KPD PREPARATION FOLLOWING CULTURING OF CL. BOTULINUM OF THE A, B, C AND E TYPES

摘要

1. During a study of changes of nitrogenous substances in a dry whale-liver medium with yeasts, their active participation was exposed in the processes of growth and toxin formation of type A, B, C and E botulism causative agents.n2. The amount of nitrogenous substances determined in the medium such as amine nitrogen, peptone, tryptophane and other amino acids, following incubation and the formation of botulin toxin of types A, B, C and E is a characteristic for each type. Thus the amount of amine nitrogen during cultivation of microbes of types A and B increased by three times, type C -- two times, and type E -- one and one half times in comparison with the original medium;the amount of peptone in the medium decreased most significantly during the growth of types A and B, less during the incubation of type C, and hardly changed following the cultivation of type E;tryptophane completely disappeared in the medium following the growth of type A and B bacilli, half the amount remained following the incubation of type C, and it was consumed insignificantly by type E.