THE DNA CONTENT OF MITOTIC NUCLEI OF TWO MOUSE TUMORS

摘要

A new high-precision microspectrophotometric method has been used to test the validity of the "DNA constancy hypothesis" in two mammalian tumor lines. The two are the mouse ascitic tumor BP-8 and the in vitro mouse leukemic cell line, L5178Y. It has been shown that the mitotic nuclei of these cells have a DNA content with a coefficient of variation of 5-6％. The instrumental and measurement errors are 4-5％;thus the variability of DNA content as measured by absorption of Feulgen stain is 1% or less.