Sugar Retention, Cofactor Levels, and Leakage of Metabolites in X-Irradiated, Starved Yeast Cells

摘要

Irradiated cells retained a greater portion of (14C)sorbose, though rates of efflux were similar during a first phase of exit and rates of uptake were similar. The thiamine pyrophosphate content of irradiated cells was greater than that of unirradiated cells, whereas pyruvate decarboxylase activities and ATP and DPN contents were similar. Similar amounts of 260 millimicrons absorbing materials were lost by irradiated and unirradiated cells during starvation. Incubation with glucose or maltose increased the total loss, while incubation with severa less readily metabolized sugars or with hexitols increased the relative loss from irradiated cells. (Author)