Studies on the Antiviral Activity of Guanylhydrazones Especially Against Arbo- and Myxoviruses

摘要

Antiviral and antitumor activities of 4,6-dichloro-2-aminophenol hydrochloride (no. 178) were studied. Non-lethal doses of no. 178 in vivo was 500 mg, 350 mg, 25 mg, and 1.5 mg per Kg. of mouse by intragastric, intraperitoneal, intranasal, and intracerebral administration, respectively. In ovo, one mg. per egg of no. 178 was non-lethal. The minimal toxic concentration of no. 178 in chorioallantoic membrane culture in vitro was 25 r/ml in final, and the minimal inhibitory concentration against influenza virus was observed as 3.2 r/ml when no. 178 was not autoclaved and 0.2 r/ml when autoclaved. Therefore, the ratio of the minimal toxic to the minimal inhibitory concentration was 8 with non-autoclaved and 125 with autoclaved solution of no. 178. The no. 178 dissolved in 50% glycerol was more effective than its distilled water solution. Three types of influenza virus, PR8 strain of type A, Adachi strain of type A2 and Lee strain of type B, and Japanese B encephalitis virus were inactivated by no. 178 solution. However, no. 178 did not inactivate polio virus containing RNA in virus and herpes simplex virus containing DNA. Cultured HeLa cell was very sensitive to no. 178 and was completely detached from glass with maintenance medium containing 25 r/ml. Ehrlich ascitic cells were relatively resistance to no. 178 and 1.5 mg/ml of no. 178 was needed to kill the cells in vitro. (Author)