Measurement of Trace Levels of Deuterium Oxide in Biologic Fluids Using Infrared Spectrophotometry

摘要

A method is described for the reliable direct quantitation of trace levels of D2O in biologic fluids without need for sample preprocessing. Experimental data relevant to the assay of D2O in human serum, urine, and parotid fluid are presented. For serum, with triplicate scans, values of precision and of accuracy of plus or minus 3% at the 250 p.p.m. D2O level are obtained. By use of parotid fluid the values are narrowed to plus or minus 2% at the 200 to 250 p.p.m. D2O range. The data also reveal good agreement between serum distillate D2O levels and concentrations calculated for serum aqueous compartments using values for serum water content. Parotid fluid appears to be particularly suitable for biomedical applications due to its ease of collection, constancy of composition, and similarity in behavior to H2O in the region of 3.98 microns. (Author)