Water Insoluble Derivatives of Proteins with Biological Activity

摘要

A number of derivatives of papain and chymotypsin were prepared in which the active enzyme was covalently bound to a high-molecular weight carrier (e.g., poly-L-ornithyl papain, poly-L-glutamyl chymotrypsin). A kinetic study was made of these compounds, with particular attention given to pH activity profiles. Papain-collodion membranes were also prepared and were examined with respect to the effect of local gradients of enzyme and substrate on their kinetic activity.