Prolongation of Allograft Survival by an Alphaglobulin Isolated from Normal Blood

摘要

A protein fraction with ability to inhibit the transplantation immune response has been isolated from normal human plasma by column chromatography, using methods similar to those previously described by Mowbray. (Transplantation 1:15, 1963) In the present experiments the active material was eluted from the column by less concentrated buffer than that employed by Mowbray. The active fraction appeared to consist largely of alphaglobulin as determined by electrophoresis. A single 100 mg. dose of the active alphaglobulin administered intravenously induced a significant (p 0.01) prolongation of survival (up to 33 days) of skin allografts in rabbits. A single intravenous infusion of 5 mg. of the alphaglobulin induced a significant (p 0.001) prolongation of survival (up to 20 days) of C57 BL skin allografts in C3H mice. The alphaglobulin appeared to be entirely nontoxic in both species in the doses employed. (Author)