Insulin-Inhibitory Factor in Human Blood: Role in the Charcoal Radioimmunoassay

摘要

The insulin-inhibitory factor in human blood was studied. The results of a recovery study with a single-antibody assay (the charcoal radioimmunoassay) were compared to those with a two-antibody assay to determine whether inhibition occurred between insulin and the first antibody or between the first and the second antibody. The single-antibody assay did not show an insulin-inhibitory factor, but the two-antibody radioimmunoassay did show an inhibitor. The inhibitor must therefore act between the anti-human (gamma globulin) antibody and the anti-insulin antibody to block the reaction. The exact nature of the inhibitor is as yet unknown. (Author)