The aim of this work was to establish a simple and reliable method for iodine labelling of different proteins and purification of the labelled products to the extent where they can be used for metabolic investigations. The pre¬paration of iodine labelled, high specific activity proteins through an electrolytical method is reported. Human serum albumin was selected as a mo¬del substance for preliminary experiments to find the optimum condition for labelling. Different proteins wereiodinated to a mean level of 0.1 - 0. 5 atom per molecule of protein, a labelling yield of ~ 90％ was achieved in most cases.nInvestigations were also made covering the purification of the labelled product immediately after the preparation by gel filtration. Metabolic studies and pyrogenity tests carried out on the labelled and purified rabbit serum al¬bumin and transferrin demonstrated that the product is pyrogene free and metabolized as the genuine proteins.
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