Inhibition and Aging of Cholinesterase in Human Red Blood Cells Poisoned in Vitro with Malathion and Malaoxon.

摘要

The effects of malathion and malaoxon on intact human red blood cells (RBC) were studied. When aging of malaoxon-inhibited cholinesterase (ChE) of intact human RBC's is studied in vitro under physiological conditions of temperature and pH, the information obtained may be directly applicable to the therapy for malaoxon poisoning. On the assumption of the same close correlation of the in vitro and in vivo rates of aging in man as has been found for different organophosphorus compounds in experimental animals, the half-time of 3.4 hours for malaoxon-inhibited ChE of human RBC's indicates the interval during which therapy with 2-PAMC1 might be effective. Intact human RBC's may slowly convert malathion to a more active metabolite. No significant inhibition of RBC ChE was obtained with malathion per se. No interfering detoxification of malaoxon by RBC's was found under conditions comparable to the inhibition step of the aging experiments. (Author)