Establishment of Three Diploid Cell Lines of Anopheles Stephensi (Diptera: Culicidae)

摘要

The establishment of three diploid cell lines of Anopheles stephensi is described. The primary cultures were initiated between February and April, 1968 and consisted of minced tissues from early first instar larvae. Trypsinized cells from such cultures readily grew when transferred to new flasks. After these initial transfers, each of the cell lines has been subcultured a minimum of 30 times. The pertinent features of the cell lines are given and some comparisons are made with respect to the other insect cell lines now available. Some observations on insect tissue culture in general are also made. (Author)