Effects of Several Stains and Fluids on Staining and Viability of Spores of Piricularia Oryzae.

摘要

Spores of Piricularia oryzae were stained with a variety of stains. Methylene Blue provided the best staining properties for observation under white light; Acridine Orange and Rhodamine B produced the better fluorescing properties. Spores of P. oryzae were also suspended in various solutions. Phloxine B inhibited spore germination completely. Cycloheximide, except at the 0.05% level and Tergitol 4, except at the 0.1% and 0.2% levels, inhibited spore germination also. Tergitol 4 stimulated germination mildly at the lower concentrations employed. To a lesser degree Rhodamine B stimulated germination at 0.05%, 0.2% and 0.1%. Methylene Blue stimulated germination at all concentrations tested except at the 3.0% level. Likewise NaC1 and Acridine Orange stimulated at all concentrations tested. Acridine Orange is recommended for staining spores to be counted for direct or automatic counting equipment. A study of persistency of P. oryzae in local soils under ambient conditions is suggested.