Comparative Study of the Haemagglutinating Arbovirus Antigens Prepared from Tissue Cultures and Mouse Brain (Sravnitelnoe Izuchenie Gemagglyutiniruyushchikh Arbovirusnykh Antigenov, Prigotovlennykh Iz Tkanevykh Kultur I Iz Mozga Myshei)

摘要

The laboratory diagnosis of the arbovirus infections and the investigation of the immunity structure of the population are restricted to a large extent by the lack of a sufficient set of non-infectious antigens for the HAI and HA tests. Up to now the most widely used method of producing the arbovirus antigens is that from mouse brain by the method of the aqueous-saline or sucrose-acetone extraction. With the development of the method of growing arboviruses in tissue cultures it became possible to obtain from this material the antigens for HAI and HA tests. The purpose of the research is the investigation of the specificity and of the activity spectrum at various pH and temperature values of the non-infectious antigens for the viruses of the Venezuelan (VEL), and Western (ZEL) equine encephalomyelitis and of Chikungunya in the HAI test. (Author)