Human Blood Stored in Cpd-Methylene Blue with Addition of Adenine and Inosine. The Effect on Hemoglobin Function, 2,3-Dpg and Atp

摘要

Addition of MB to CPD blood in concentrations of 0.00001 M in the presence of 0.5 mM adenine resulted in ATP levels consistently higher than the corresponding controls and almost double after 4 and 6 weeks of storage. Under the same conditions, the levels of 2,3-DPG showed a decrease during the first 3 weeks of storage, but rose above the control values at the fourth week and were three times higher after 6 weeks of storage. Addition of 15 mM inosine in the presence of 0.00001 and 0.000001 M MB resulted in slightly lowered ATP values, but vastly increased 2,3-DPG values compared to the controls throughout the 6 weeks storage time. Compared to the controls, hemoglobin function was better maintained by the addition of adenine in the presence of 0.00001 and 0.000001 M MB. In the presence of inosine and 0.00001 and 0.000001 M MB, hemoglobin function remained normal throughout the 6 weeks storage time. (Author)