Mouse Model for Aerosol Infection of Influenza (Postprint).

摘要

To provide a method of detecting clinical response as a complement to a bioaerosol delivery system (CATS) designed to measure infective penetration of respiratory protective devices, an animal model system was developed for measuring the infectivity of aerosol challenges delivered to mice. The CATS was used to deliver graduated doses of influenza A/PR/8/34 (H1N1) by inhalation to 25-g female CD-1 mice, from which a median infective dose (MID50) of less than 40 TCID50 was inferred from weight loss, and a lower- limit value of approximately 12 TCID50 was calculated from cytopathic effect and qRT-PCR data. The information derived from this study can be used to further the development of mouse models for evaluating aerosol delivery of pharmaceuticals and technologies for infectious disease control, and to create and validate computational models for aerosol delivery.