The Relationship between Hemoglobin Function and Membrane Integrity in Erythrocytes during Cold Storage

摘要

Whole human blood with and without the addition of adenine-inosine was stored for 42 days. Adenine-inosine was effective in maintaining hemoglobin function as shown by a continued high level of 2,3-DPG. Analysis of the erythrocyte membranes was carried out by chemical and physicochemical methods. It was shown that adenine-inosine treatment does not alter the rate of membrane deterioration, indicating that the maintenance of membrane integrity and hemoglobin function is a separate problem and each must be treated individually. The determining factor in long-term red cell preservation may well reside in membrane stabilization. (Author)