Nutrient (Lipid, Nucleoside, and Soluble Metabolite) Studies on Intraerythrocytic Malaria Parasites

摘要

The sum of various efforts to culture malaria during more than half a century suggests that achievement of sustained in vitro cultivation requires a confluence of expertise from the basic disciplines that underlie the field of parasitology. The present study is concerned with advances in intracellular cultivation along lines where further progress appears to be feasible. More than one generation of malaria can be raised in red cells. There is considerable information available regarding physiology of blood cells and biochemistry of the plasma in which they are suspended. The essential and novel item of the new system is a rotary connector. This links six lines that transmit liquids and gases to and from culture chambers spinning in a centrifuge. Preliminary tests of the first model indicated that the design selected is feasible. A revision needed for initiation of cultivation studies was under construction at the end of the report period. (Author)