Uptake and Retention of Adenine Moiety by Stored Human Red Blood Cells

摘要

The adenine moiety, which is utilized by the red cells for net synthesis of various nucleotides, does not appear to be in a steady-state equilibrium between plasma and erythrocytes. As storage time increases, the equilibrium is shifted towards the red cells and reached a maximum radioactive content at 21 days. After this time a reversal occurs. At the beginning of storage the association of adenine with erythrocytes is not very strong, since the radioactive material can be removed to a great extent by saline extraction. The retention increases with length of storage and attains a maximum value at 21 days. Specific association of the radioactive label has been observed with cell populations of low density (young cells). (Author)